Spinal Cord Injury (SCI) can result in catastrophic loss of function. In the US, 450,000 people live with SCI. Ongoing neuroscience research focuses on ways to improve nervous tissue regeneration, including development of innovative biomaterials. Implantable scaffolds composed of aligned polymer fibers have shown considerable promise in directing regenerating axons in vitro and in vivo. Highly aligned polymer fibers are necessary for neural tissue engineering applications to ensure that axonal extension occurs efficiently through a regenerating environment. This technology is directed to an implantable scaffold and a method of making that scaffold using highly aligned electrospun polymer fibers in a multilayered three-dimensional implantable structure that retains the fiber alignment. The scaffold includes a generally circular outer profile and a selection of inner profiles, such as those with S- W-, Z-shapes, etc. The scaffold is particularly advantageous for use as for tissue regeneration, especially nervous tissue regeneration. Animal models of nerve injury that were implanted with the scaffold showed robust guided axonal regeneration compared to control.