The creation of miniature biochemical analysis systems using microfavrication technology is a recent significant development in the field of micofluidics. These systems offer advantages such as size reduction, power reduction, and increased reliability. However, current systems are tailored to a specific task, and thus are unable to be reconfigured and reprogramed to handly a variety of tasks.

While there have been rapid advances in microscale device fabrication, microfluidics, and lab-on-a-chip technology, there is still a need to immobilize biomolecules (especially proteins) on a microfluidics apparatus, while maintaining high biological activity and electroosmotic flow (EOF) capability. This invention relates to the discovery that certain polymers containing both ionic and hydrophobic groups can be reproducibly adhered to microfluidic channels and can be used to simulataneously immobilize biocatalysts with good catalytic activity while supporting EOF.