This invention is directed to a high-throughput process for screening proteins for kinetic stability.Kinetically stable proteins are trapped in one conformation and have a high barrier to unfolding, so they are resistant to aggregation and degradation and have a longer half-life.The ability to quickly and easily identify kinetically stable proteins would have a myriad of applications in the biotechnology industry, pharmaceutical industry, and in basic life science research.

While there have been rapid advances in microscale device fabrication, microfluidics, and lab-on-a-chip technology, there is still a need to immobilize biomolecules (especially proteins) on a microfluidics apparatus, while maintaining high biological activity and electroosmotic flow (EOF) capability. This invention relates to the discovery that certain polymers containing both ionic and hydrophobic groups can be reproducibly adhered to microfluidic channels and can be used to simulataneously immobilize biocatalysts with good catalytic activity while supporting EOF.