This invention is directed to a high-throughput process for screening proteins for kinetic stability.Kinetically stable proteins are trapped in one conformation and have a high barrier to unfolding, so they are resistant to aggregation and degradation and have a longer half-life.The ability to quickly and easily identify kinetically stable proteins would have a myriad of applications in the biotechnology industry, pharmaceutical industry, and in basic life science research. The current invention identifies such proteins more quickly and easily than existing methods, employing mutant generation, SDS degradation tests, and 2-D electrophoresis gels.Applications for the method include protein therapeutic drugs (because of long shelf life), biosensors, and biomaterials.

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Natasha Sanford