Displacement chromatography has attracted signifcant attention as a powerful technique for the purification of bioherapeutic proteins and oligunucleotides. Displacement chromatography enables simultaneous concentration and purification in a single step, which is significant in the purifcation of biopharmaceuticals. However, the major obstacle in implementing this technique is the lack of a sufficient diversity of appropriate displace candidates that are applicable across a wide spectrum of bioseparation demands.

After discovering that a natural product has a particular, useful biological activity, it is desirable to prepare analogs of the natural product. However, natural products and their analogs are typically complex molecules requiring multi-step syntheses that are usually laborious, costly, and time consuming. This invention is directed to a microfluidics device that can be used to prepare natural products and their analogs.

This invention is directed to a step-wise enzymatic synthesis of combinatorial libraries of polymeric compounds prepared on a solid support in a configuration suitable for high-throughput screening for use in drug discovery and related fields. This invention provides compositions and methods for generating and screening libraries of phenolicanilinic polymers (and their related quinine forms) attached to a suitable surface and synthesized using enzymatic catalysis.

Ultrafiltration (UF) membranes have found widespread use in the food and biotechnology industries. UF has been applied in the processing of normal and transgenic milk, cheese and eggs, whey and potato protein recovery, the clarification of juices and wine, the recovery of proteins from animal blood, and the purification of water. UF is also used in the biotechnology industry for the recovery of biological products through such steps as cell broth clarification, cell harvesting, concentration or diafiltration of protein solutions prior to separation, and final concentration.

Displacement chromatography is a powerful preparative technique that can offer high production rates, resolving power and elevated yields and purity of a desired byproduct. Historically, it was necessary to screen displacer candidates individually in column experiments using trial and error, a very laborious task.